AGX51 for Dummies
The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items have been digested and cloned into a pET21a+ vector employing NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the short improve site-directed mutagenesis method u